Ⅰ型前胶原氨基端原肽(PINP)检测河北快三走势图表(酶联免疫吸附试验法,快捷型)

Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide

  • 编号IEA957Ra
  • 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
  • 所有
  • 小鼠
  • 大鼠
  • 豚鼠
  • 家兔
  • 猕猴
  • 山羊
  • 绵羊
  • 其他物种
  • 多物种
  • 泛物种
  • 实验方法双抗夹心
  • 反应时长1h, 10min
  • 检测范围246.9-20,000pg/mL
  • 灵敏度最小可检测剂量小于等于92.4pg/mL.
  • 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • 下载 英文说明书   中文说明书
  • 规格 48T96T 96T*5 96T*10 96T*100
  • 价格 ¥ 4309 ¥ 6156 ¥ 27702 ¥ 52326 ¥ 430920
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  • Ⅰ型前胶原氨基端原肽(PINP)检测河北快三走势图表(酶联免疫吸附试验法,快捷型) 产品包装(模拟)
  • Ⅰ型前胶原氨基端原肽(PINP)检测河北快三走势图表(酶联免疫吸附试验法,快捷型) 产品包装(模拟)
  • Ⅰ型前胶原氨基端原肽(PINP)检测河北快三走势图表(酶联免疫吸附试验法,快捷型) 实验结果图
  • IEA957Ra.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证
  • 特异性

    本河北快三走势图表用于检测Ⅰ型前胶原氨基端原肽(PINP)检测河北快三走势图表(酶联免疫吸附试验法,快捷型),经检测与其它相似物质无明显交叉反应。
    由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本河北快三走势图表有可能与未经检测的其它物质有交叉反应。

    回收率

    分别于定值血清及血浆样本中加入一定量的Ⅰ型前胶原氨基端原肽(PINP)检测河北快三走势图表(酶联免疫吸附试验法,快捷型)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

    样本
    回收率范围(%)
    平均回收率(%)
    serum(n=5)
    83-103
    98
    EDTA plasma(n=5)
    93-101
    96
    heparin plasma(n=5)
    90-98
    94

    精密度

    精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
    批内差:取同批次河北快三走势图表对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
    批间差:选取3个不同批次的河北快三走势图表分别对低、中、高值定值样本进行定量测定,每个样本使用同一河北快三走势图表重复测定8次,分别计算不同浓度样本的平均值及SD值。
    批内差: CV<10%
    批间差: CV<12%

    线性

    在定值血清及血浆样本内加入适量的Ⅰ型前胶原氨基端原肽(PINP)检测河北快三走势图表(酶联免疫吸附试验法,快捷型),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中Ⅰ型前胶原氨基端原肽(PINP)检测河北快三走势图表(酶联免疫吸附试验法,快捷型)含量的测定值与理论值的比率。

    样本
    1:2
    1:4
    1:8
    1:16
    serum(n=5)
    88-95%
    78-88%
    94-102%
    92-101%
    EDTA plasma(n=5)
    93-102%
    85-92%
    78-91%
    95-103%
    heparin plasma(n=5)
    82-102%
    80-93%
    85-95%
    99-105%

    稳定性

    经测定,河北快三走势图表在有效期内按推荐温度保存,其活性降低率小于5%。
    为减小外部因素对河北快三走势图表破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

    实验流程

    1. 实验前标准品、试剂及样本的准备;
    2. 加样(标准品及样本)50µL后,立即加入检测溶液A50µL,
        37°C孵育50分钟;
    3. 甩干,洗板3次;
    4. 加检测溶液B100µL, 37°C孵育10分钟;
    5. 洗板5次;
    6. 加TMB底物90µL,37°C孵育10-20分钟;
    7. 加终止液50µL,立即450nm读数。

    实验原理

    将Ⅰ型前胶原氨基端原肽(PINP)检测河北快三走势图表(酶联免疫吸附试验法,快捷型)单克隆抗体包被于96孔微孔板中,制成固相载体,向微孔中加入标准品或标本以及同时加入HRP标记Ⅰ型前胶原氨基端原肽(PINP)检测河北快三走势图表(酶联免疫吸附试验法,快捷型)的多克隆抗体,孵育结束彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的被检测物浓度呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

    赠品

    96孔板检测数据分析软件

    增值服务

    检测河北快三走势图表单组分试剂
    ELISA/CLIA实验服务

    相关产品

    编号
    适用物种:Rattus norvegicus (Rat,大鼠)
    应用(仅供研究使用,不用于临床诊断!)
    RPA957Ra01
    Ⅰ型前胶原氨基端原肽(PINP)重组蛋白
    Positive Control; Immunogen; SDS-PAGE; WB.
    CPA957Ra21
    Ⅰ型前胶原氨基端原肽(PINP)卵白蛋白偶联物
    Immunogen; SDS-PAGE; WB.
    PAA957Ra01
    Ⅰ型前胶原氨基端原肽(PINP)多克隆抗体
    WB; IHC; ICC; IP.
    LAA957Ra71
    Ⅰ型前胶原氨基端原肽(PINP)多克隆抗体(生物素标记)
    WB; IHC; ICC.
    SEA957Ra
    Ⅰ型前胶原氨基端原肽(PINP)检测河北快三走势图表(酶联免疫吸附试验法)
    Enzyme-linked immunosorbent assay for Antigen Detection.
    IEA957Ra
    Ⅰ型前胶原氨基端原肽(PINP)检测河北快三走势图表(酶联免疫吸附试验法,快捷型)
    Enzyme-linked immunosorbent assay for Antigen Detection.
    CEA957Ra
    Ⅰ型前胶原氨基端原肽(PINP)检测河北快三走势图表(酶联免疫吸附试验法)
    Enzyme-linked immunosorbent assay for Antigen Detection.
    KSA957Ra11
    Ⅰ型前胶原氨基端原肽(PINP)检测河北快三走势图表DIY材料(酶联免疫吸附试验法)
    Main materials for "Do It (ELISA Kit) Yourself".

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