超氧化物歧化酶(SOD)检测河北快三走势图表(酶联免疫吸附试验法)

ELISA Kit for Superoxide Dismutases (SOD)

  • 编号SES134Mu
  • 物种Mus musculus (Mouse,小鼠) 相同的名称,不同的物种。
  • 所有
  • 小鼠
  • 大鼠
  • 豚鼠
  • 家兔
  • 猕猴
  • 山羊
  • 绵羊
  • 其他物种
  • 多物种
  • 泛物种
  • 实验方法双抗夹心
  • 反应时长3h
  • 检测范围0.156-10ng/mL
  • 灵敏度最小可检测剂量小于等于0.060ng/mL.
  • 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • 下载 英文说明书   中文说明书
  • 规格 48T96T 96T*5 96T*10 96T*100
  • 价格 ¥ 3326 ¥ 4752 ¥ 21384 ¥ 40392 ¥ 332640
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  • 超氧化物歧化酶(SOD)检测河北快三走势图表(酶联免疫吸附试验法) 产品包装(模拟)
  • 超氧化物歧化酶(SOD)检测河北快三走势图表(酶联免疫吸附试验法) 产品包装(模拟)
  • 超氧化物歧化酶(SOD)检测河北快三走势图表(酶联免疫吸附试验法) 实验结果图
  • SES134Mu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证
  • 特异性

    本河北快三走势图表用于检测超氧化物歧化酶(SOD),经检测与其它相似物质无明显交叉反应。
    由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本河北快三走势图表有可能与未经检测的其它物质有交叉反应。

    回收率

    分别于定值血清及血浆样本中加入一定量的超氧化物歧化酶(SOD)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

    样本
    回收率范围(%)
    平均回收率(%)
    serum(n=5)
    78-105
    91
    EDTA plasma(n=5)
    90-98
    94
    heparin plasma(n=5)
    84-104
    101

    精密度

    精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
    批内差:取同批次河北快三走势图表对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
    批间差:选取3个不同批次的河北快三走势图表分别对低、中、高值定值样本进行定量测定,每个样本使用同一河北快三走势图表重复测定8次,分别计算不同浓度样本的平均值及SD值。
    批内差: CV<10%
    批间差: CV<12%

    线性

    在定值血清及血浆样本内加入适量的超氧化物歧化酶(SOD),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中超氧化物歧化酶(SOD)含量的测定值与理论值的比率。

    样本
    1:2
    1:4
    1:8
    1:16
    serum(n=5)
    83-97%
    80-97%
    82-94%
    78-91%
    EDTA plasma(n=5)
    79-97%
    80-99%
    88-95%
    97-105%
    heparin plasma(n=5)
    86-93%
    89-103%
    87-94%
    79-103%

    稳定性

    经测定,河北快三走势图表在有效期内按推荐温度保存,其活性降低率小于5%。
    为减小外部因素对河北快三走势图表破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

    实验流程

    1. 实验前标准品、试剂及样本的准备;
    2. 加样(标准品及样本)100µL,37°C孵育1小时;
    3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
    4. 洗板3次;
    5. 加检测溶液B100µL,37°C孵育30分钟;
    6. 洗板5次;
    7. 加TMB底物90µL,37°C孵育10-20分钟;
    8. 加终止液50µL,立即450nm读数。

    实验原理

    将超氧化物歧化酶(SOD)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的超氧化物歧化酶(SOD)与连接于固相载体上的抗体结合,然后加入生物素化的超氧化物歧化酶(SOD)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的超氧化物歧化酶(SOD)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

    赠品

    96孔板检测数据分析软件
    辣根过氧化物酶标记的链霉亲和素

    增值服务

    检测河北快三走势图表单组分试剂
    酶联免疫河北快三走势图表定制服务
    ELISA/CLIA实验服务
    体外诊断(IVD)试剂原料——抗原
    体外诊断(IVD)试剂原料——抗体

    相关产品

    编号
    适用物种:Mus musculus (Mouse,小鼠)
    应用(仅供研究使用,不用于临床诊断!)
    SES134Mu
    超氧化物歧化酶(SOD)检测河北快三走势图表(酶联免疫吸附试验法)
    Enzyme-linked immunosorbent assay for Antigen Detection.

    参考文献

    杂志
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