脂联素(ADPN)检测河北快三走势图表(酶联免疫吸附试验法)

ELISA Kit for Adiponectin (ADPN)

GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1

  • 编号SEA605Bo
  • 物种Bos taurus; Bovine (Cattle,牛) 相同的名称,不同的物种。
  • 所有
  • 小鼠
  • 大鼠
  • 豚鼠
  • 家兔
  • 猕猴
  • 山羊
  • 绵羊
  • 其他物种
  • 多物种
  • 泛物种
  • 实验方法双抗夹心
  • 反应时长3h
  • 检测范围0.78-50ng/mL
  • 灵敏度最小可检测剂量小于等于0.33ng/mL.
  • 样本类型serum, plasma and other biological fluids
  • 下载 英文说明书   中文说明书
  • 规格 48T96T 96T*5 96T*10 96T*100
  • 价格 ¥ 3175 ¥ 4536 ¥ 20412 ¥ 38556 ¥ 317520
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  • 脂联素(ADPN)检测河北快三走势图表(酶联免疫吸附试验法) 产品包装(模拟)
  • 脂联素(ADPN)检测河北快三走势图表(酶联免疫吸附试验法) 产品包装(模拟)
  • 脂联素(ADPN)检测河北快三走势图表(酶联免疫吸附试验法) 实验结果图
  • SEA605Bo.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证
  • 特异性

    本河北快三走势图表用于检测脂联素(ADPN),经检测与其它相似物质无明显交叉反应。
    由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本河北快三走势图表有可能与未经检测的其它物质有交叉反应。

    回收率

    分别于定值血清及血浆样本中加入一定量的脂联素(ADPN)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

    样本
    回收率范围(%)
    平均回收率(%)
    serum(n=5)
    78-89
    84
    EDTA plasma(n=5)
    96-104
    101
    heparin plasma(n=5)
    97-105
    101

    精密度

    精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
    批内差:取同批次河北快三走势图表对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
    批间差:选取3个不同批次的河北快三走势图表分别对低、中、高值定值样本进行定量测定,每个样本使用同一河北快三走势图表重复测定8次,分别计算不同浓度样本的平均值及SD值。
    批内差: CV<10%
    批间差: CV<12%

    线性

    在定值血清及血浆样本内加入适量的脂联素(ADPN),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中脂联素(ADPN)含量的测定值与理论值的比率。

    样本
    1:2
    1:4
    1:8
    1:16
    serum(n=5)
    83-101%
    87-95%
    82-103%
    78-96%
    EDTA plasma(n=5)
    96-105%
    94-101%
    95-104%
    89-101%
    heparin plasma(n=5)
    81-92%
    95-103%
    80-88%
    93-102%

    稳定性

    经测定,河北快三走势图表在有效期内按推荐温度保存,其活性降低率小于5%。
    为减小外部因素对河北快三走势图表破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

    实验流程

    1. 实验前标准品、试剂及样本的准备;
    2. 加样(标准品及样本)100µL,37°C孵育1小时;
    3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
    4. 洗板3次;
    5. 加检测溶液B100µL,37°C孵育30分钟;
    6. 洗板5次;
    7. 加TMB底物90µL,37°C孵育10-20分钟;
    8. 加终止液50µL,立即450nm读数。

    实验原理

    将脂联素(ADPN)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的脂联素(ADPN)与连接于固相载体上的抗体结合,然后加入生物素化的脂联素(ADPN)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的脂联素(ADPN)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

    赠品

    96孔板检测数据分析软件

    增值服务

    检测河北快三走势图表单组分试剂
    ELISA/CLIA专用裂解液
    ELISA/CLIA质控品
    酶联免疫河北快三走势图表定制服务
    人类疾病动物模型定制服务
    血清定制服务
    ELISA/CLIA实验服务
    TGFB1活化剂
    实时荧光定量PCR实验服务

    相关产品

    编号
    适用物种:Bos taurus; Bovine (Cattle,牛)
    应用(仅供研究使用,不用于临床诊断!)
    RPA605Bo01
    脂联素(ADPN)重组蛋白
    Positive Control; Immunogen; SDS-PAGE; WB.
    PAA605Bo01
    脂联素(ADPN)多克隆抗体
    WB; IHC; ICC; IP.
    MAA605Bo21
    脂联素(ADPN)单克隆抗体
    WB; IHC; ICC; IP.
    SEA605Bo
    脂联素(ADPN)检测河北快三走势图表(酶联免疫吸附试验法)
    Enzyme-linked immunosorbent assay for Antigen Detection.

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    杂志
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