白介素18(IL18)检测河北快三走势图表(化学发光免疫分析法)

CLIA Kit for Interleukin 18 (IL18)

IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma

  • 编号SCA064Ra
  • 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
  • 所有
  • 小鼠
  • 大鼠
  • 豚鼠
  • 家兔
  • 猕猴
  • 山羊
  • 绵羊
  • 其他物种
  • 多物种
  • 泛物种
  • 实验方法双抗夹心
  • 反应时长2h, 40min
  • 检测范围2.74-2,000pg/mL
  • 灵敏度最小可检测剂量小于等于1.22pg/mL.
  • 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • 下载 英文说明书   中文说明书
  • 规格 48T96T 96T*5 96T*10 96T*100
  • 价格 ¥ 3830 ¥ 5472 ¥ 24624 ¥ 46512 ¥ 383040
  • 欲了解实际交易价格和更多情况,请与当地经销商联系!
  • 白介素18(IL18)检测河北快三走势图表(化学发光免疫分析法) 产品包装(模拟)
  • 白介素18(IL18)检测河北快三走势图表(化学发光免疫分析法) 产品包装(模拟)
  • 白介素18(IL18)检测河北快三走势图表(化学发光免疫分析法) 实验结果图
  • SCA064Ra.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证
  • 特异性

    本河北快三走势图表用于检测白介素18(IL18),经检测与其它相似物质无明显交叉反应。
    由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本河北快三走势图表有可能与未经检测的其它物质有交叉反应。

    回收率

    分别于定值血清及血浆样本中加入一定量的白介素18(IL18)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

    样本
    回收率范围(%)
    平均回收率(%)
    serum(n=5)
    79-101
    92
    EDTA plasma(n=5)
    95-103
    101
    heparin plasma(n=5)
    88-96
    92

    精密度

    精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
    批内差:取同批次河北快三走势图表对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
    批间差:选取3个不同批次的河北快三走势图表分别对低、中、高值定值样本进行定量测定,每个样本使用同一河北快三走势图表重复测定8次,分别计算不同浓度样本的平均值及SD值。
    批内差: CV<10%
    批间差: CV<12%

    线性

    在定值血清及血浆样本内加入适量的白介素18(IL18),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中白介素18(IL18)含量的测定值与理论值的比率。

    样本
    1:2
    1:4
    1:8
    1:16
    serum(n=5)
    87-104%
    94-102%
    80-91%
    82-91%
    EDTA plasma(n=5)
    91-104%
    80-90%
    90-102%
    88-95%
    heparin plasma(n=5)
    87-94%
    91-105%
    93-104%
    90-97%

    稳定性

    经测定,河北快三走势图表在有效期内按推荐温度保存,其活性降低率小于5%。
    为减小外部因素对河北快三走势图表破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

    实验流程

    1. 实验前标准品、试剂及样本的准备;
    2. 加样(标准品及样本)100µL,37°C孵育1小时;
    3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
    4. 洗板3次;
    5. 加检测溶液B100µL,37°C孵育30分钟;
    6. 洗板5次;
    7. 加底物100µL,37°C孵育10分钟;
    8. 读数。

    实验原理

    将白介素18(IL18)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的白介素18(IL18)与连接于固相载体上的抗体结合,然后加入生物素化的白介素18(IL18)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的白介素18(IL18)呈正相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

    赠品

    96孔板检测数据分析软件

    增值服务

    检测河北快三走势图表单组分试剂
    ELISA/CLIA专用裂解液
    ELISA/CLIA质控品
    化学发光河北快三走势图表定制服务
    人类疾病动物模型定制服务
    血清定制服务
    ELISA/CLIA实验服务
    TGFB1活化剂
    实时荧光定量PCR实验服务
    体外诊断(IVD)试剂原料——抗原
    体外诊断(IVD)试剂原料——抗体

    相关产品

    编号
    适用物种:Rattus norvegicus (Rat,大鼠)
    应用(仅供研究使用,不用于临床诊断!)
    RPA064Ra01
    白介素18(IL18)重组蛋白
    Positive Control; Immunogen; SDS-PAGE; WB.
    PAA064Ra01
    白介素18(IL18)多克隆抗体
    WB; IHC; ICC; IP.
    LAA064Ra71
    白介素18(IL18)多克隆抗体(生物素标记)
    WB; IHC; ICC.
    MAA064Ra21
    白介素18(IL18)单克隆抗体
    WB; IHC; ICC; IP.
    MEA064Ra
    白介素18(IL18)检测河北快三走势图表(酶联免疫吸附试验法,小样本)
    Enzyme-linked immunosorbent assay for Antigen Detection.
    SEA064Ra
    白介素18(IL18)检测河北快三走势图表(酶联免疫吸附试验法)
    Enzyme-linked immunosorbent assay for Antigen Detection.
    SCA064Ra
    白介素18(IL18)检测河北快三走势图表(化学发光免疫分析法)
    Chemiluminescent immunoassay for Antigen Detection.

    参考文献

    杂志
    参考文献
    International Immunopharmacology
    Curcumin immune-mediated and anti-apoptotic mechanisms protect against renal ischemia/reperfusion and distant organ induced injuries [ScienceDirect: S1567576911001068]
    Atherosclerosis
    IL-18 overexpression promotes vascular inflammation and remodeling in a rat model of metabolic syndrome [ScienceDirect: S0021915009006200]
    PLoS One.
    HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa Keratitis [Plos: Source]
    临床儿科杂志
    中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18 在诊断心肺分流术后急性肾损伤中的意义 [Jcp-Sh:Source]
    Cell Death Dis.
    MicroRNA-30d regulates cardiomyocyte pyroptosis by directly targeting foxo3a in diabetic cardiomyopathy [Pubmed:Pmc4237254]
    PLoS One.
    Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion [Pubmed:24816434]
    J Korean Med Sci.
    Acute Kidney Injury after Using Contrast during Cardiac Catheterization in Children with Heart Disease [Pubmed:25120320]
    PLOS ONE
    Protective Effect of Leaf Essential Oil from [PubMed: 25794175]
    Fertil Steril
    Inflammosome in the human endometrium: further step in the evaluation of the “maternal side” [PubMed: 26474737]
    United States Patent Application
    GLUCAN-ENCAPSULATED SIRNA FOR TREATING TYPE 2 DIABETES MELLITUS [y2016:0138028.html]
    Front Pharmacol
    Poria Attenuates Idiosyncratic Liver Injury Induced by Polygoni Multiflori Radix Praeparata [pubmed:27803670]
    Pharmacological reports
    The Role of PON-1, GR, IL-18, and oxLDL in Depression With and Without Posttraumatic Stress Disorder [science:article]
    International Journal of Molecular Sciences
    PEDF Inhibits the Activation of NLRP3 Inflammasome in Hypoxia Cardiomyocytes through PEDF Receptor/Phospholipase A2 [pubmed:27973457]
    Cellular Physiology and Biochemistry
    MiR-155 is Involved in Renal Ischemia-Reperfusion Injury via Direct Targeting of FoxO3a andRegulating Renal Tubular Cell Pyroptosis. [pubmed:28006785]
    Journal of Food and Drug Analysis
    Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum Kanehira in endotoxin-induced … [S1021-9498(17)30081-9]
    Journal of Microbiology, Immunology and Infection
    Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients [pubmed:28655573]
    Discovery and Validation of Immunological Biomarkers in Milk for Health Monitoring of Dairy Cows - Results from a Multiomics Approach [doi:10.4172/2329-888X.1000182]
    Cellular Physiology and Biochemistry
    Dexmedetomidine Alleviates HyperoxiaInduced Acute Lung Injury via Inhibiting NLRP3 Inflammasome Activation [pubmed:28873369]
    20
    Melatonin prevents endothelial cell pyroptosis via regulation of long noncoding RNA MEG3/miR-223/NLRP3 axis [pubmed:29024030]
    Pharmacological Reports
    The role of PON-1, GR, IL-18, and OxLDL in depression with and without posttraumatic stress disorder. [pubmed:28623707]
    World journal of gastroenterology
    Hypothermic machine perfusion with metformin-University of Wisconsin solution for ex vivo preservation of standard and marginal liver grafts in a rat model. [pubmed:29142469]
    Veterinary microbiology
    Secondary Haemophilus parasuis infection enhances highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection-mediated inflammatory responses. [pubmed:28532803]
    International immunopharmacology
    Chronic dexamethasone treatment results in hippocampal neurons injury due to activate NLRP1 inflammasome in vitro [pubmed:28605710]
    Journal of Neuroinflammation
    Chronic glucocorticoid exposure activates BK-NLRP1 signal involving in hippocampal neuron damage. [pubmed:28732502]
    Journal of Neuroinflammation
    Unconjugated bilirubin induces pyroptosis in cultured rat cortical astrocytes [Pubmed:29357878]
    Oxidative Medicine and Cellular Longevity
    The Effects of Sinapic Acid on the Development of Metabolic Disorders Induced by Estrogen Deficiency in Rats [Pubmed:29967666]
    Journal of Translational Medicine
    Recurrent pregnancy loss is associated to leaky gut: a novel pathogenic model of endometrium inflammation? [Pubmed:29665864]
    Chemico-Biological Interactions
    Nifuroxazide, a STAT3 inhibitor, mitigates inflammatory burden and protects against diabetes-induced nephropathy in rats [Pubmed:29291386]
    Journal of Food and Drug Analysis
    Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum Kanehira in endotoxin-induced mice [Pubmed:29389558]
    International Journal of Molecular Medicine
    MCMV triggers ROS/NLRP3-associated inflammasome activation in the inner ear of mice and cultured spiral ganglion neurons, contributing to sensorineural hearing … [Pubmed:29512778]
    Archives of Virology
    Genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central China [Pubmed:29305646]
    Journal of Inflammation
    Expression and clinical significance of the NEK7-NLRP3 inflammasome signaling pathway in patients with systemic lupus erythematosus [Pubmed: 30202244]
    Journal of Affective Disorders
    Post-stroke depression and cognitive impairment: study design and preliminary findings in a Brazilian prospective stroke cohort (EMMA study) [Doi: 10.1016/j.jad.2018.10.003]
    BioScientia Medicina
    Sensitivity and Spesificity of Interleukin 18 as Biomarker for Acute Kidney Injury []
    International Journal of Molecular Medicine
    Ginsenoside Rg1 protects against H2O2‑induced neuronal damage due to inhibition of the NLRP1 inflammasome signalling pathway in hippocampal neurons in vitro [Pubmed: 30535505]
    Nutrients
    Effect of Rosmarinic Acid on the Serum Parameters of Glucose and Lipid Metabolism and Oxidative Stress in Estrogen-Deficient Rats [Pubmed: 30691017]
    International immunopharmacology
    NADPH oxidase 2-mediated NLRP1 inflammasome activation involves in neuronal senescence in hippocampal neurons in vitro [Pubmed: 30677569]
    Acta Histochemica
    Pre-administration of luteoline attenuates neonatal sevoflurane-induced neurotoxicity in mice [Doi: 10.1016/j.acthis.2019.04.004]
    JOURNAL OF DRUG TARGETING
    Artemisinin alleviates atherosclerotic lesion by reducing macrophage inflammation via regulation of AMPK/NF-κB/NLRP3 inflammasomes pathway [Pubmed: 31094238]
    IUBMB Life
    Melatonin Enhances Autophagy and Decreases Apoptosis Induced by nanosilica in RAW264. 7 cells [Pubmed: 31018046]
    American Journal of Reproductive Immunology
    Effect of alpha lipoic acid and myoinositol on endometrial inflammasome from recurrent pregnancy loss women [Pubmed: 31148259]
    INFLAMMATION RESEARCH
    TNF-α/calreticulin dual signaling induced NLRP3 inflammasome activation associated with HuR nucleocytoplasmic shuttling in rheumatoid arthritis [Pubmed: 31119302]
    PLoS One
    Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model [Pubmed: 31247004]
    留言咨询